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In Vitro Fertilization of Cougar Using Preserved Genetic Material

Updated: Feb 18



onça-parda macho atropelado, oócitos vitrificados
Road-killed donor (left), vitrified oocytes after rewarming (top right), and rescued testicles from the run-over male (bottom right).

Researchers conducted a pioneering study involving in vitro fertilization (IVF) using vitrified oocytes and semen collected from the epididymis of a run-over cougar (Puma concolor). This research highlights the feasibility of preserving genetic material from endangered animals, even after death, which aids conservation strategies for the species.


The study was conducted in collaboration between Brazilian and Canadian scientists and involved collecting semen from a male hit by a vehicle. After obtaining the material, it was stored and prepared for IVF. Previously collected and vitrified oocytes were used in the procedure.


During fertilization, an initial embryonic structure was observed, though embryo development was interrupted at early stages. The conservation of endangered species relies on the continued advancement of reproductive techniques. Studies like this demonstrate biotechnology's potential to mitigate the impacts of individual losses in nature and ensure the genetic diversity of wild populations.


Check out the full study:



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© 2025 Instituto Reprocon. 

The Reprocon Institute is a OSCIP (Civil Society Organization in the Public Interest), registered under CNPJ No. 39.247.082/0001-09 and legally constituted as a private non-profit civil association. Its headquarters is located in the municipality of Campo Grande, Mato Grosso do Sul, Brazil.

Reprocon (Reproduction for Conservation) is also certified by DGP-CNPq as a research group.

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